WHAT IS RAJATA BHASMA LIQUID?

Bringing Nanotechnology – The GREEN Way

Silver Nanoparticles

Gold Nanoparticles

Why us?

STUDIES & RESULTS

About 5% concentration is very effective in Destroying Microbes (99.9999%) within contact time of 1 min

S. No.

Microbe

Silver Nanoparticles (Rajata Bhasma Liquid)

 
 
1 min
Log red.
5 min
Log red.
10 min
Log red.
1
E. coli
0
6.0
0
6.0
0
6.0
2
S. aureus
0
6.0
0
6.0
0
6.0
3
P. aeruginosa
0
6.0
0
6.0
0
6.0
4
C. albicans
0
6.0
0
6.0
0
6.0
5
A. brasiliensis
73
3.1
62
3.2
41
3.4
  • Initial microbial load is 106 CFU/ml.
  • Acceptance criteria for disinfectant is 3.0 log reduction.
  • About 5% concentration is very effective in destroying microbes (99.9999%) within contact time of 1 min
  • 50% of these organisms were destroye in <1 min contact time.

Major Ingredients used in Anti-microbials and their efficacy

Group

Gram Positive

Bacteria

Gram Negative

Bacteria

Bacteria

Fungi

Viruses

Speed of Action

  Comments

Alcohols

+++

+++

+++

+++

+++

Fast

Optimum concentration 60%-95%; no persistent activity

Chlorhexidine (2% and 4% aqueous)

+++

++

+

+

+++

Intermediate

Persistent activity; rare allergic reactions

Iodine compounds

+++

+++

+++

++

+++

Intermediate

Causes skin burns; Intermediate usually too irritating for hand hygiene

Iodophors

+++

+++

+

++

++

Intermediate

Less irritating than iodine; acceptance varies

Phenol derivatives

+++

+

+

+

+

Intermediate

Activity neutralized by nonionic surfactants

Triclosan

+++

++

+

+++

Intermediate

Acceptability on hands varies

Quaternary ammonium

+

++

+

Slow

Used only in combination with alcohols; ecological concerns

Rajata Bhasma Liquid – Silver Nanoparticles (8.8ppm and 43.8ppm)

+++

+++

+++

+++

++

Fast

Persistent activity; Penetrates Bioflims

and Non Toxic

Note: +++ = excellent; == good, but does not include the entire bacterial spectrum. + = fair, – – + no activity or not sufficient. *Hexachlorophene is not included because it is no longer an acceptable ingredient.

Anti-microbial efficacy of Rajata Bhasma Liquid

Microorganism

ATCC Number

MIC (g/mL)

MBC (g/mL)

EC50 (g/mL)

Escherichia Coli

12435

5

10

3.5

Pseudomonas aeruginosa

15442

5

5

3

Enterobacter aerogenes

13048

10

10

4

Acinectobacteria baumannii

19606

5

10

2

Staphylococcus aureus

25923

10

>20

4

Staphylococcus spidermidis

35984

5

20

4

Bacillus subtitis

6051

5

20

2

Entercococcus faecalis

29212

5

5

3

Candida albicans

10231

10

5

>0.16

MIC – Minimum Inhibitory Concentration: Lowest concentration of an anti-bacterial agent necessary to inhibit visible growth

MBC – Minimum Bactericidal Concentration: Lowest concentration of Silver Nanoparticles to prevent growth.

EC50 – Concentration of a drug that gives half-maximal response.

Toxicity studies of Rajata Bhasma Liquid

Toxicity Tests

Guidelines

Results

Single dose oral toxicity (Sprague Dawley Rats and Swiss Albino Mice)

As per Schedule Y

LD50 is > 10 ml/kg body weights

Repeated dose toxicity for 28 days. (Sprague Dawley Rats and New Zealand White Rabbits)

As per Schedule Y

LD50 is>10 ml/kg body weight

Acute eye irritation study (New Zealand White Rabbits)

Organization for Economic Co-operation and Development (OECD)

Non-irritant

Note: Each ml of Rajata Bhasma Liquid contains Silver equivalent 180 - 220 mcg.

Sanitizer Germ Kill Efficacy (Time Kill Assay Method)

Procedure:

  • Antimicrobial effectiveness tested for the different Sanitizers 6% Rajata Bhasma Liquid by time kill Assay method (ASTM 2315)

Interpretation:

  • 3 to 4 log reduction seen in 10sec, 30sec, 1min and 5min contact time for all bacteria.
  • 4 log reduction seen in 10sec, 30sec, 1mon and 5min contact time for yeast and molds
  • Overall, 99.9% reduction of microorganisms seen in all three different products
    of sanitizer.

Evaluation of Skin irritation potential of Rajata Bhasma Liquid Sanitizer

Protocol:

  • Dermatological evaluation of hand sanitizer+ by single occlusive patch test on healthy adult human volunteers (24 human subjects)

 

           The effect of the product was evaluated on 3-day’s period and follow up visit at T8 (T+1 week after 0 hours of patch removal).

RESULTS :

  • Non-Irritant to Skin
  • No adverse effects (erythema, itching, irritation, rashes, stinging) were observed

Published Literature: Established Efficacy of Rajata Bhasma (Nanosilver) against viruses.

Herpes Case Study with Rajata Bhasma Liquid - vishari

Medical Condition

Herpes

Age / Gender

50 Y / Male

Diagnosed on

2015 and taking allopathy treatment for 5 years

Nanolife Treatment Duration

20 days

Treated with

Vishari 10ml twice daily (10ml – 0 – 10ml) for 20 days

Antiviral Potential of Silver nanoparticles References:

Silver Nanoparticles as Potential Antiviral Agents, Molecules 2011, 16, 8894-8918; doi:10.3390/molecules16108894

Mode of antiviral action of silver nanoparticles against HIV-1. J Nanobiotechnology 8, 1 (2010). https://doi.org/10.1186/1477-3155-8-1

An overview application of silver nanoparticles in inhibition of herpes simplex virus, Artificial Cells, Nanomedicine, and Biotechnology, 46:2, 263-267, DOI: 10.1080/21691401.2017.1307208

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